Position Specifics:

South San Francisco, CA

3T Biosciences is solving a major bottleneck in the field of immunotherapy – identifying novel targets that can be used to generate therapies to treat cancer in broad patient populations. We are developing transformative T cell receptor therapies for cancer and other immune-related diseases. Our proprietary experimental and computational technology allows us to identify novel T cell receptor targets as well as clinical toxicities to bring safe and effective therapies.


We’re committed to making a difference for patients. Our focused, creative, and ambitious team makes 3T Biosciences the perfect engine to drive therapeutic solutions to reality. We’re looking for enthusiastic and self-motivated individuals to bring their talents to a fast-paced environment.

Your Typical Responsibilities:

  • Engineer primary human T cells and immortalized cell lines for expression of therapeutic TCR candidates using viral and non-viral methods
  • Conceive of and implement genome engineering strategies to support internal discovery research efforts; optimize new and existing processes for cell yield, purity, phenotype, and function
  • As needed, collaborate with other scientists and research associates to carry out functional assays with engineered T cells (cytokine secretion, cytotoxicity, etc.)

Required Qualifications:

  • PhD plus 0-4 years additional experience in academia or industry
  • Experience engineering primary T cells using lentivirus, CRISPR/Cas9, or related methods
  • Experience with flow cytometry, including panel design and data analysis
  • Highly motivated and proactive; able to work independently and in teams
  • Very organized, with excellent time management
  • Superior written and verbal scientific communication skills

Preferred Qualifications:

  • 2+ years industry experience
  • Experience using and optimizing CRISPR/Cas9-mediated knock-in for transgene expression in primary T cells
  • Direct experience with CAR-T or TCR-T cells, and familiarity with critical parameters that impact their efficacy and persistence
  • Experience using automation for multiplexed engineering and purification of mammalian cells
  • Experience using Benchling and Asana


Please contact john.leonard@3tbiosciences.com with your cover letter and resume.

Please contact info@3tbiosciences.com with your cover letter and resume.